Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007-2008 respiratory season.
Identifieur interne : 000712 ( Main/Exploration ); précédent : 000711; suivant : 000713Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007-2008 respiratory season.
Auteurs : A. Eshaghi [Canada] ; S. Bolotin ; L. Burton ; D E Low ; T. Mazzulli ; S J DrewsSource :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [ 1873-5967 ] ; 2009.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Analyse de séquence d'ADN, Antiviraux (pharmacologie), Données de séquences moléculaires, Enfant, Enfant d'âge préscolaire, Femelle, Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains, Jeune adulte, Mutation faux-sens, Mâle, Nourrisson, Ontario (épidémiologie), Oséltamivir (pharmacologie), Polymorphisme génétique, Protéines virales (génétique), Résistance virale aux médicaments, Sialidase (génétique), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (isolement et purification), Substitution d'acide aminé (génétique), Sujet âgé, Sujet âgé de 80 ans ou plus.
- MESH :
- génétique : Protéines virales, Sialidase, Sous-type H1N1 du virus de la grippe A, Substitution d'acide aminé.
- isolement et purification : Sous-type H1N1 du virus de la grippe A.
- pharmacologie : Antiviraux, Oséltamivir.
- virologie : Grippe humaine.
- épidémiologie : Grippe humaine, Ontario.
- Adolescent, Adulte, Adulte d'âge moyen, Analyse de séquence d'ADN, Données de séquences moléculaires, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mutation faux-sens, Mâle, Nourrisson, Polymorphisme génétique, Résistance virale aux médicaments, Sujet âgé, Sujet âgé de 80 ans ou plus.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Amino Acid Substitution (genetics), Antiviral Agents (pharmacology), Child, Child, Preschool, Drug Resistance, Viral, Female, Humans, Infant, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza, Human (epidemiology), Influenza, Human (virology), Male, Middle Aged, Molecular Sequence Data, Mutation, Missense, Neuraminidase (genetics), Ontario (epidemiology), Oseltamivir (pharmacology), Polymorphism, Genetic, Sequence Analysis, DNA, Viral Proteins (genetics), Young Adult.
- MESH :
- chemical , genetics : Neuraminidase, Viral Proteins.
- chemical , pharmacology : Antiviral Agents, Oseltamivir.
- geographic , epidemiology : Ontario.
- epidemiology : Influenza, Human.
- genetics : Amino Acid Substitution, Influenza A Virus, H1N1 Subtype.
- isolation & purification : Influenza A Virus, H1N1 Subtype.
- virology : Influenza, Human.
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Drug Resistance, Viral, Female, Humans, Infant, Male, Middle Aged, Molecular Sequence Data, Mutation, Missense, Polymorphism, Genetic, Sequence Analysis, DNA, Young Adult.
Abstract
BACKGROUND
The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance.
OBJECTIVES
To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis.
STUDY DESIGN
Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using chi(2) analyses.
RESULTS
The HA gene of influenza virus A (H1N1) isolates collected during the 2007-2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 (chi(2)=284.9, df=2, p<0.0001).
CONCLUSIONS
During the 2007-2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein.
DOI: 10.1016/j.jcv.2009.04.010
PubMed: 19451021
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Mazzulli</name></author><author><name sortKey="Drews, S J" sort="Drews, S J" uniqKey="Drews S" first="S J" last="Drews">S J Drews</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19451021</idno><idno type="pmid">19451021</idno><idno type="doi">10.1016/j.jcv.2009.04.010</idno><idno type="wicri:Area/Main/Corpus">000771</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000771</idno><idno type="wicri:Area/Main/Curation">000771</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000771</idno><idno type="wicri:Area/Main/Exploration">000771</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007-2008 respiratory season.</title><author><name sortKey="Eshaghi, A" sort="Eshaghi, A" uniqKey="Eshaghi A" first="A" last="Eshaghi">A. Eshaghi</name><affiliation wicri:level="1"><nlm:affiliation>Ontario Public Health Laboratories, 81 Resources Road, Toronto, Ontario, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ontario Public Health Laboratories, 81 Resources Road, Toronto, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Bolotin, S" sort="Bolotin, S" uniqKey="Bolotin S" first="S" last="Bolotin">S. Bolotin</name></author><author><name sortKey="Burton, L" sort="Burton, L" uniqKey="Burton L" first="L" last="Burton">L. Burton</name></author><author><name sortKey="Low, D E" sort="Low, D E" uniqKey="Low D" first="D E" last="Low">D E Low</name></author><author><name sortKey="Mazzulli, T" sort="Mazzulli, T" uniqKey="Mazzulli T" first="T" last="Mazzulli">T. Mazzulli</name></author><author><name sortKey="Drews, S J" sort="Drews, S J" uniqKey="Drews S" first="S J" last="Drews">S J Drews</name></author></analytic><series><title level="j">Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</title><idno type="eISSN">1873-5967</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Amino Acid Substitution (genetics)</term><term>Antiviral Agents (pharmacology)</term><term>Child</term><term>Child, Preschool</term><term>Drug Resistance, Viral</term><term>Female</term><term>Humans</term><term>Infant</term><term>Influenza A Virus, H1N1 Subtype (genetics)</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza, Human (epidemiology)</term><term>Influenza, Human (virology)</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Mutation, Missense</term><term>Neuraminidase (genetics)</term><term>Ontario (epidemiology)</term><term>Oseltamivir (pharmacology)</term><term>Polymorphism, Genetic</term><term>Sequence Analysis, DNA</term><term>Viral Proteins (genetics)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Analyse de séquence d'ADN</term><term>Antiviraux (pharmacologie)</term><term>Données de séquences moléculaires</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Grippe humaine (virologie)</term><term>Grippe humaine (épidémiologie)</term><term>Humains</term><term>Jeune adulte</term><term>Mutation faux-sens</term><term>Mâle</term><term>Nourrisson</term><term>Ontario (épidémiologie)</term><term>Oséltamivir (pharmacologie)</term><term>Polymorphisme génétique</term><term>Protéines virales (génétique)</term><term>Résistance virale aux médicaments</term><term>Sialidase (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term><term>Substitution d'acide aminé (génétique)</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Oseltamivir</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Ontario</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amino Acid Substitution</term><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Sialidase</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Substitution d'acide aminé</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Oséltamivir</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term><term>Ontario</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Child</term><term>Child, Preschool</term><term>Drug Resistance, Viral</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Mutation, Missense</term><term>Polymorphism, Genetic</term><term>Sequence Analysis, DNA</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mutation faux-sens</term><term>Mâle</term><term>Nourrisson</term><term>Polymorphisme génétique</term><term>Résistance virale aux médicaments</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b></p><p>To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis.</p></div><div type="abstract" xml:lang="en"><p><b>STUDY DESIGN</b></p><p>Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using chi(2) analyses.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The HA gene of influenza virus A (H1N1) isolates collected during the 2007-2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 (chi(2)=284.9, df=2, p<0.0001).</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>During the 2007-2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19451021</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>10</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-5967</ISSN><JournalIssue CitedMedium="Internet"><Volume>45</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</Title><ISOAbbreviation>J. Clin. Virol.</ISOAbbreviation></Journal><ArticleTitle>Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007-2008 respiratory season.</ArticleTitle><Pagination><MedlinePgn>142-5</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jcv.2009.04.010</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis.</AbstractText><AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using chi(2) analyses.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The HA gene of influenza virus A (H1N1) isolates collected during the 2007-2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 (chi(2)=284.9, df=2, p<0.0001).</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">During the 2007-2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Eshaghi</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Ontario Public Health Laboratories, 81 Resources Road, Toronto, Ontario, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bolotin</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Burton</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Low</LastName><ForeName>D E</ForeName><Initials>DE</Initials></Author><Author ValidYN="Y"><LastName>Mazzulli</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Drews</LastName><ForeName>S J</ForeName><Initials>SJ</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>FJ800809</AccessionNumber><AccessionNumber>FJ800810</AccessionNumber><AccessionNumber>FJ800811</AccessionNumber><AccessionNumber>FJ800812</AccessionNumber><AccessionNumber>FJ800813</AccessionNumber><AccessionNumber>FJ800814</AccessionNumber><AccessionNumber>FJ800815</AccessionNumber><AccessionNumber>FJ800816</AccessionNumber><AccessionNumber>FJ800817</AccessionNumber><AccessionNumber>FJ800818</AccessionNumber><AccessionNumber>FJ800819</AccessionNumber><AccessionNumber>FJ800820</AccessionNumber><AccessionNumber>FJ800821</AccessionNumber><AccessionNumber>FJ800822</AccessionNumber><AccessionNumber>FJ800823</AccessionNumber><AccessionNumber>FJ800824</AccessionNumber><AccessionNumber>FJ800825</AccessionNumber><AccessionNumber>FJ800826</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>05</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Clin Virol</MedlineTA><NlmUniqueID>9815671</NlmUniqueID><ISSNLinking>1386-6532</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>20O93L6F9H</RegistryNumber><NameOfSubstance UI="D053139">Oseltamivir</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber><NameOfSubstance UI="C487630">NA protein, influenza A virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber><NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019943" MajorTopicYN="N">Amino Acid Substitution</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D024882" MajorTopicYN="Y">Drug Resistance, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020125" MajorTopicYN="Y">Mutation, Missense</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009439" MajorTopicYN="N">Neuraminidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009864" MajorTopicYN="N" Type="Geographic">Ontario</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053139" MajorTopicYN="N">Oseltamivir</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011110" MajorTopicYN="Y">Polymorphism, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>03</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>04</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>5</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>5</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>7</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19451021</ArticleId><ArticleId IdType="pii">S1386-6532(09)00170-X</ArticleId><ArticleId IdType="doi">10.1016/j.jcv.2009.04.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Bolotin, S" sort="Bolotin, S" uniqKey="Bolotin S" first="S" last="Bolotin">S. Bolotin</name><name sortKey="Burton, L" sort="Burton, L" uniqKey="Burton L" first="L" last="Burton">L. Burton</name><name sortKey="Drews, S J" sort="Drews, S J" uniqKey="Drews S" first="S J" last="Drews">S J Drews</name><name sortKey="Low, D E" sort="Low, D E" uniqKey="Low D" first="D E" last="Low">D E Low</name><name sortKey="Mazzulli, T" sort="Mazzulli, T" uniqKey="Mazzulli T" first="T" last="Mazzulli">T. Mazzulli</name></noCountry><country name="Canada"><noRegion><name sortKey="Eshaghi, A" sort="Eshaghi, A" uniqKey="Eshaghi A" first="A" last="Eshaghi">A. Eshaghi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007-2008 respiratory season.
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